產(chǎn)品描述
四環(huán)素陰性胎牛血清(tetracycline negative fetal bovine serum)胎牛血清一般包含抗生素四環(huán)素(tet),當(dāng)使用非常敏感的tet-誘導(dǎo)的基因表達(dá)系統(tǒng)時(shí),研究人員會(huì)面臨意料之外的表達(dá)問(wèn)題。血清含有zui低級(jí)別的四環(huán)素是必要的,以確保精確的基因表達(dá)。胎牛血清會(huì)含有四環(huán)素,科研工作者在使用敏感四環(huán)素誘導(dǎo)調(diào)控表達(dá)系統(tǒng)時(shí),往往會(huì)遇到非預(yù)期基因表達(dá)的問(wèn)題。為確保精確的基因表達(dá),血清中所含的四環(huán)素越少越好。這款產(chǎn)品可應(yīng)用于使用敏感四環(huán)素誘導(dǎo)表達(dá)系統(tǒng)的細(xì)胞培養(yǎng),以及其它對(duì)四環(huán)素敏感的實(shí)驗(yàn)中。四環(huán)素檢測(cè)含量約為0.001µg/ml。
產(chǎn)品特點(diǎn)
quality profile
specification
result
unit
appearance
clear amber
pass
ph
6.8-8.2
7.30
osmolality
280-340
315
mosm/kg
endotoxin
as reported
1.01
ng/ml
hemoglobin
≤25
14.9
mg/ml
tetracycline/-derivates
≤0.5
pass
μg/ml
glucose
as reported
3.87
mm
calcium
as reported
2.65
mm
phosphorous
as reported
2.79
mm
got/ast
as reported
40
u/l
ldh
as reported
599
u/l
protein profile
total protein
3.0-4.5
3.7
g/dl
albumin
as reported
2.2
g/dl
lgg
≤250.0
189.00
mg/l
sterility testing
bactria and fungi
not detected
not detected
mycoplasma
not detected
not detected
virus testing
bovine herpes virus(bhv-1 and bhv-5)
not detected
not detected
pestivirus(e.g.bvd-v)
not detected
not detected
parainfluenza virus type 3(pi-3)
not detected
not detected
biological performance
cell growth mdck(madin-darby canine kidney line)
≥75.0
101.0
%cell growth cho(chinese hamster ovary cell line)
≥75.0
100.0
%
產(chǎn)品應(yīng)用
四環(huán)素陰性胎牛血清四環(huán)素陰性胎牛血清常用于科研、診斷試劑、疫苗生產(chǎn)和研發(fā),同時(shí)也能夠用于作為免疫反應(yīng)中的阻斷劑和蛋白配體反應(yīng)中的固定劑。
運(yùn)輸與保存方法
干冰運(yùn)輸,-20℃保存,有效期5年。
注意事項(xiàng)注意事項(xiàng)
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本產(chǎn)品主要用于科研領(lǐng)域,不宜用于臨床診斷或其他用途。
貨號(hào) 品名 規(guī)格 品牌
78ed10009-500ml 四環(huán)素陰性胎牛血清 500ml biohub
78ed10009-10*50 ml 四環(huán)素陰性胎牛血清 10*50 ml biohub
78ed10009-50ml 四環(huán)素陰性胎牛血清 50ml biohub