血紅素加氧酶-1 (ho-1)也稱為hsp32，是血紅素加氧酶的誘導型異構(gòu)體，對血紅素的nadph、氧和細胞色素p450還原酶依賴性氧化進行催化，氧化為一氧化碳、亞鐵和膽綠素，膽綠素迅速還原為膽紅素。這些反應產(chǎn)物具有重要的生理作用：一氧化碳是一種有效的血管擴張劑，并被認為是cgmp和血管緊張度的生理調(diào)節(jié)劑；膽綠素及其產(chǎn)物膽紅素是有效的抗氧化劑；“游離”鐵通過影響鐵調(diào)節(jié)蛋白(irp)-1的構(gòu)象及其與mrnas的5’-或3’-utr中的鐵調(diào)節(jié)元件(ires)的結(jié)合，增加氧化應激并調(diào)節(jié)許多mrnas(例如，dct-1、鐵蛋白和轉(zhuǎn)移受體)的表達。三種已鑒定的血紅素加氧酶亞型是催化血紅素轉(zhuǎn)化為膽綠素和一氧化碳的血紅素加氧酶系統(tǒng)的一部分，分別是可誘導的ho-1或hsp32，在腦和睪丸中大量存在的組成型ho-2，以及與ho-2相關(guān)但為不同基因產(chǎn)物的ho-3。
enzo life sciences的ho-1 (human), elisa kit提供了一種檢測和定量人源樣品中血紅素氧合酶(ho-1)的方法。該試劑盒可以精確地測定細胞裂解物（cell lysates）和組織提取物（tissue extracts）中的ho-1，不與人ho-2或ho-3(其他已知的血紅素加氧酶同種型)交叉反應。
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產(chǎn)品特點
● 高靈敏度，可檢測低至0.78 ng/ml的ho-1
● 重復可靠的檢測結(jié)果
● 高通量，可在2.5h小時內(nèi)完成多達40個樣本的檢測（含復孔）
● 優(yōu)于半定量western blot分析的全定量檢測
產(chǎn)品信息
產(chǎn)品貨號 adi-eks-800
產(chǎn)品名稱
ho-1 (human), elisa kit（人血紅素加氧酶-1 elisa試劑盒）
規(guī)格
96 wells
別名
heme oxygenase 1
靈敏度
0.78 ng/ml
檢測范圍
0.78 - 25 ng/ml
檢測時間
<2.5 hours
適用種屬
human
交叉反應
human ho-1 (100%). no reactivity with ho-2 or ho-3.
適用樣本類型
for the quantitative determination of human heme oxygenase-1 in
cell lysates and tissue extracts. cited sample types include serum
and plasma.
試劑盒組分
microtiter plate, 5x extraction reagent, standard, sample diluent,
wash buffer concentrate, antibody, antibody diluent, hrp conjugate,
conjugate diluent, tmb substrate, stop solution 2
標準曲線示例
部分產(chǎn)品文獻引用
1. does a negative correlation of heme oxygenase-1 with hematoma thickness in chronic subdural hematomas affect neovascularization and microvascular leakage? a retrospective study with preliminary validation: h.t. luh, et al.; j. neurosurg. (2023)
2. delivery of self-replicating messenger rna into the brain for the treatment of ischemic stroke: m. kim, et al.; j. control release 350, 471 (2022)
3. effects of tolvaptan on oxidative stress in adpkd: a molecular biological approach: m. rigato, et al.; j. clin. med. 11, 402 (2022)
4. associations between blood heavy metal(loid)s and serum heme oxygenase-1 in pregnant women: do their distribution patterns matter?: k. li, et al.; environ. pollut. 286, 117249 (2021)
5. dual-functional dendrimer micelles with glycyrrhizic acid for anti-inflammatory therapy of acute lung injury: m. choi, et al.; acs appl. mater. interfaces 13, 47313 (2021)
6. induction of ho-1 by 5, 8-dihydroxy-4,7-dimethoxyflavone via activation of ros/p38 mapk/nrf2 attenuates thrombin-induced connective tissue growth factor expression in human cardiac fibroblasts: c. yang, et al.; oxid. med. cell. longev. 2020, 1080168 (2020)
7.beneficial use of serum ferritin and heme oxygenase-1 as biomarkers in adult-onset still's disease: a multicenter retrospective study: y. kirino, et al.; mod. rheumatol. 28, 858 (2018), application(s): elisa using human serum
8. camel milk ameliorates 5-fluorouracil-induced renal injury in rats: targeting mapks, nf-κb and pi3k/akt/enos pathways: h.h. arab, et al.; cell. physiol. biochem. 46, 1628 (2018), application(s): elisa with rat
9. elisa development for serum hemeoxygenase-1 and its application to patients with acute respiratory distress syndrome: y. hara, et al.; can. respir. j. 2018, 9627420 (2018), application(s): human serum
10. endothelin-1-induced hypertrophic alterations and heme oxygenase-1 expression in cardiomyoblasts are counteracted by beta estradiol: in vitro and in vivo studies: t. barta, et al.; naunyn schmiedebergs arch. pharmacol. 391, 371 (2018)
11. haemolysis and haem oxygenase-1 induction during persistent 'asymptomatic' malaria infection in burkinabé children: j.p. mooney, et al.; malar. j. 17, 253 (2018)
12. hepatoprotective potential of standardized ficus species in intrahepatic cholestasis rat model: involvement of nuclear factor-κb, and farnesoid x receptor signaling pathways: s.s. el-hawary, et al.; j. ethnopharmacol. 231, 262 (2018)
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