the detection of foodborne bacteria on beef: theapplication of the electronic nose
電子鼻用于牛肉食源性細(xì)菌檢測(cè)
soad a abdallah, laila a al-shatti, ali f alhajraf, noura al-hammad, bashayer al-awadi
abstract
this study aims to investigate the application of a fast electronic nose system (cyranose 320) for detecting foodborne bacteria. the system proved to be very efficient in detecting microbes in beef and sausage samples. in the first part of the study, the total viable counts (tvc) from fresh and frozen beef samples were determined using the standard microbiological method and by the application of the electronic nose. the second part applied the electronic nose to beef before and after contamination with different bacterial pathogens separay: e. coli o157: h7, salmonellatyphimurium 857, staphylococcus aureusand pseudomonas aeruginosa 27853. the results revealed that the cyranose 320 can detect the tvc in different beef and sausage samples and quantify the volatile organic compounds produced at concentrations from 50 ppb to > 350 ppb. the concentrations of gases collected from the samples before and after separate contamination with these pathogenic bacteria were highly significantly correlated (p < 0.005). from this study one can conclude that the electronic nose system is a rapid way for detecting volatile organic compounds produced by foodborne bacteria that contaminate beef.
本研究旨在探討快速電子鼻系統(tǒng)(cyranose320)在食源性細(xì)菌檢測(cè)中的應(yīng)用。該系統(tǒng)對(duì)牛肉和香腸樣品中的微生物檢測(cè)非常有效。在研究的部分，新鮮和冷凍牛肉樣品的總活菌計(jì)數(shù)(tvc)是通過標(biāo)準(zhǔn)微生物法和電子鼻的應(yīng)用來確定的。第二部分分別將電子鼻應(yīng)用于牛肉被不同細(xì)菌性病原體污染前后：大腸桿菌o157:h7、沙門氏菌857、*29213、銅綠假單胞菌27853。結(jié)果表明，cyranose320可檢測(cè)不同牛肉和香腸樣品中的tvc，并可定量測(cè)定濃度為50 ppb至>350 ppb的揮發(fā)性有機(jī)化合物。與這些病原菌分離污染前后從樣品中收集的氣體濃度高度相關(guān)(p<0.005)。從這項(xiàng)研究可以得出結(jié)論，電子鼻系統(tǒng)是一種快速檢測(cè)揮發(fā)性有機(jī)化合物產(chǎn)生的食源性細(xì)菌污染牛肉。
keywords:food, pathogens, rapid detection, electronic nose