applications reacts specifically and rapidly with thiols to form mixed disulfides. used to probe the structures of the ach receptor channel of the gaba receptor channel and of lactose permease.
中文名：[2-(氨基羰基)乙基] 甲烷硫代磺酸酯
英文名：mtsace
[2-(aminocarbonyl)ethyl]methanethiosulfonate
methanesulfonothioic acid s-(3-amino-3-oxopropyl) ester
cas：351422-28-1
分子式：c4h9no3s2
分子量：183.25
儲存條件： 吸濕，-20°c冰箱，惰性氣體下
some methanethiosulfonates are hygroscopic and all hydrolyze in water, over a period of time, particularly in the presence of nucleophiles. they should be stored in a desiccator at minus 20 degree c and warmed up to room temperature before opening the vial. for maximum results, solutions should be made up immediately prior to use even though solutions in distilled water appear to be stable for hours at 4 degree c.
at ph 7.5 and room temperature, mtsea hydrolyzes with a half-life of about 15 minutes, mtset hydrolyzes with a half-life of 10 minutes, and mtses hydrolyzes with a half-life of 20 minutes. routinely, one can use 2.5mm mtsea, 1mm mtset, or 10mm mtses, applied for 1 to 5 minutes. (mtset is 2.5 times as reactive with small sulfhydryl compounds as is mtsea, and 10 times as reactive as mtses). (stauffer and karlin, 1994; karlin, personal communication).
for application to xenopus oocytes, dissolve the compounds in 115mm nacl, 2.5mm kcl, 1.8mm mgcl2, 10mm hepes, ph 7.5 with naoh. mts reagents decompose in buffer very quickly.
dmso is a good solvent for the mts reagents which are not water soluble (i.e. the non-charged mts reagents).
其他相關(guān)試劑：
385398-78-7 mtsbs
385398-80-1 mtspes
385398-83-4 mtsps
386229-78-3 mts-teah
219789-15-8 mts-ptrea
386229-80-7 alpha-環(huán)檸檬醛，alpha-cyclociral
386229-81-8 mtsmt
220560-60-1 mtspt
1219378-82-1 2-carboxyethyl methanethiosulfonate, choline ester chloride salt
351422-29-2 cys-mts
14202-77-8 allyl methanethiosulfonate
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