nmt是基因功能的活體檢測(cè)技術(shù)，已被31位諾貝爾獎(jiǎng)得主所在單位，及北大、清華、中科院使用。?
nmt歷*的今天
2016年11月13日，中科院煙臺(tái)海岸帶研究所駱永明、李連禎利用nmt在plant and soil 上發(fā)表了標(biāo)題為determining the fluxes of ions (pb2+, cu2+ and cd2+) at the root surface of wetland plants using the scanning ion-selective electrode technique的研究成果。
期刊：plant and soil
主題：使用掃描離子選擇電極技術(shù)確定濕地植物根表面離子（pb2+，cu2+和cd2+）的通量
標(biāo)題：determining the fluxes of ions (pb2+, cu2+ and cd2+) at the root surface of wetland plants using the scanning ion-selective electrode technique
影響因子：3.410
檢測(cè)指標(biāo)：pb2+、cu2+、cd2+流速
作者：中科院煙臺(tái)海岸帶研究所駱永明、李連禎
英文摘要
measuring specific ion fluxes from different regions of the root under practical physiological conditions is crucial for understanding metal uptake mechanisms by plants.
we developed and tested a neutral carrier-based liquid-membrane pb2+ and cu2+ ion selective microelectrode (isme) to investigate ion-transport processes along the roots of three common wetland plant species.
the pb2+ and cu2+ isme exhibited a nernstian response with pb2+ and cu2+ activities as low as 1.0 nm and 1.0 μm in deionized water and simulated soil solution, respectively. phragmites australis had a region of cu2+ release for approximately the first 200 μm, while it exhibited pb2+ and cd2+ outward net flux up to the first 500 μm. although in older sections of the root of phragmites australis there were areas of influx of cu2+, pb2+ and cd2+, the overall influx was much smaller than that of typha latifolia or canna indica. such a reduced uptake and/or an increased efflux of metal ions across the root-cell plasma-membrane might explain the higher resistance of phragmites australis to metals, at least in part.
the pb2+ and cu2+ ismes are shown to permit detailed investigation of heavy-metal ion transport in plant roots, especially for plants used for phytoremediation.
中文摘要（谷歌機(jī)翻）
在實(shí)際的生理?xiàng)l件下，測(cè)量來(lái)自根不同區(qū)域的比離子通量對(duì)于理解植物對(duì)金屬的吸收機(jī)理至關(guān)重要。
我們開(kāi)發(fā)并測(cè)試了基于中性載體的液膜pb2+和cu2+離子選擇性微電極（isme），以研究沿三種常見(jiàn)濕地植物物種根部的離子遷移過(guò)程。
在去離子水和模擬土壤溶液中，pb2+和cu2+的isme表現(xiàn)出nernstian響應(yīng)，pb2+和cu2+的活性分別低至1.0 nm和1.0μm。蘆葦在大約初的200μm處具有cu2+釋放區(qū)域，而在初的500μm處表現(xiàn)出pb2+和cd2+向外凈通量。盡管在蘆葦根部的較老部分中有cu2+，pb2+和cd2+的流入?yún)^(qū)域，但總流入量比香蒲或印度香豆小得多。穿過(guò)根細(xì)胞質(zhì)膜的這種減少的金屬離子吸收和/或增加的金屬離子外流可能至少部分解釋了蘆葦對(duì)金屬的更高抗性。
研究表明，pb2+和cu2+ isme可以詳細(xì)研究植物根特別是用于植物修復(fù)的植物中重金屬離子的轉(zhuǎn)運(yùn)。
fig. 3 measurement of fluxes (outward positive) of cd2+,pb2+ and cu2+ (mean ± standard error) across the root tips of three common wetland plant species, typha latifolia (a) canna indic (b) and phragmites australis (c), using cd2+,pb2+ and cu2+ ion selective microelectrodes and the scanning ion-selective electrode higher metal influx in the root regions, especially in the meristematic and elongation zone (fig. 3).