epicypher是一家為表觀遺傳學(xué)和染色質(zhì)生物學(xué)研究提供高質(zhì)量試劑和工具的專業(yè)制造商。epicypher推出的cut&run級別的ctcf antibody符合epicypher的“cutana compatible”標(biāo)準，可用于核酸酶靶向切割和釋放（cut&run）和/或靶向剪切及轉(zhuǎn)座酶技術(shù)（cut&tag）的基因組定位。ctcf通過結(jié)合dna并阻止啟動子和附近增強子或沉默子之間的相互作用來調(diào)節(jié)轉(zhuǎn)錄。
產(chǎn)品詳情
反應(yīng)種屬:human, mouse
宿主來源:rabbit
實驗應(yīng)用:cut&run, ihc, icc, ip, wb
免疫原:between amino acids 650 and 700
克隆性:monoclonal [blr041f]
表達系統(tǒng):hek293 cells
保存溫度:stable for 1 year at 4°c from date of receipt
運輸溫度:do not freeze!! frozen cold packs.
產(chǎn)品形式:purified recombinant monoclonal antibody in borate buffered saline ph 8.2, 0.1% bsa, 0.09% sodium azide
數(shù)據(jù)示例
figure 1:ctcf peaks in cut&run
cut&run was performed as described above. peaks were called with macs2. heatmaps show ctcf peaks relative to igg negative control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. ctcf showed expected enrichment around the tss. despite some observable background in the igg control, differential enrichment with the target antibody is as expected.
figure 2: ctcf cut&run representative browser tracks
cut&run was performed as described above. gene browser shots were generated using the integrative genomics viewer (igv, broad institute). three representative loci are shown.
figure 3: immunohistochemistry data
ffpe sections of human colon carcinoma (left) and mouse spleen (right) using ctcf antibody at a dilution of 1:1000.
figure 4: immunocytochemistry data
ffpe section of human mcf-7 cells examined using ctcf antibody at a dilution of 1:1,000.
figure 5: immunoprecipitation data
epicypher ctcf antibody (6μg/mg lysate) was used to immunoprecipitate whole cell lysates (1 mg, 20% of ip loaded) isolated from hek293t cells. a negative control igg antibody and positive control antibodies (bethyl laboratories) were also used to demonstrate specificity of the ip. all ctcf antibodies target the same ctcf epitope, however, a300-534a-6 is hosted in rabbit. for blotting immunoprecipitates, epicypher ctcf antibody was used at a 1:1,000 dilution.
figure 6: western blot data
western analysis of ctcf in whole cell extracts from hek293t, mcf-7, jurkat, hep-g2, and a-549 cells. fifty micrograms of lysate was resolved via sds-page and detected with a 1:1,000 dilution of ctcf antibody.
訂購詳情
貨號
產(chǎn)品名稱
規(guī)格
13-2014
ctcf cutana™ cut&run antibody
100 µl