product details
the 10f.9g2 monoclonal antibody reacts with mouse pd-l1 (programmed death ligand 1) also known as b7-h1 or cd274. pd-l1 is a 40 kda type i transmembrane protein that belongs to the b7 family of the ig superfamily. pd-l1 is expressed on t lymphocytes, b lymphocytes, nk cells, dendritic cells, as well as ifnγ stimulated monocytes, epithelial cells and endothelial cells. pd-l1 binds to its receptor, pd-1, found on cd4 and cd8 thymocytes as well as activated t and b lymphocytes and myeloid cells. engagement of pd-l1 with pd-1 leads to inhibition of tcr-mediated t cell proliferation and cytokine production. pd-l1 is thought to play an important role in tumor immune evasion. induced pd-l1 expression is common in many tumors and results in increased resistance of tumor cells to cd8 t cell mediated lysis. in mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between pd-l1 and pd-1. the 10f.9g2 antibody has been shown to block the interaction between pd-l1 and pd-1 and between pd-l1 and b7-1 (cd80).
bioxcell艾美捷be0101體內(nèi)單克隆抗小鼠pd-l1(b7-h1)抗體:
10f.9g2單克隆抗體與小鼠pd-l1（程序性死亡配體1）反應(yīng)，也稱為b7-h1或cd274。pd-l1是一種40 kda的i型跨膜蛋白，屬于ig超家族的b7家族。pd-l1在t淋巴細胞，b淋巴細胞，nk細胞，樹突狀細胞以及ifnγ刺激的單核細胞，上皮細胞和內(nèi)皮細胞上表達。pd-l1與其受體pd-1結(jié)合，pd-4存在于cd8和cd1胸腺細胞以及活化的t和b淋巴細胞和骨髓細胞上。
bioxcell艾美捷體內(nèi)單克隆抗小鼠pd-l1(b7-h1)抗體規(guī)格：
# bxc-be0101
isotyperat igg2b, κ
recommended isotype control(s)invivomab rat igg2b isotype control, anti-keyhole limpet hemocyanin
recommended dilution bufferinvivopure ph 6.5 dilution buffer
immunogenmouse cd274
reported applicationsin vivo pd-l1 blockade
immunofluorescence
immunohistochemistry (frozen)
flow cytometry
western blot
formulationpbs, ph 6.5
contains no stabilizers or preservatives
endotoxin<2eu/mg (<0.002eu/μg)
determined by lal gel clotting assay
purity>95%
determined by sds-page
sterility0.2 μm filtered
productionpurified from tissue culture supernatant in an animal free facility
purificationprotein g
rridab_10949073
molecular weight150 kda
storagethe antibody solution should be stored at the stock concentration at 4°c. do not freeze.
同種型大鼠igg2b， κ
推薦的同種型對照體內(nèi)mab大鼠igg2b同種型對照，抗鎖孔帽貝血藍蛋白
推薦的稀釋緩沖液體內(nèi)純ph 6.5 稀釋緩沖液
免疫原鼠標cd274
報告的應(yīng)用程序體內(nèi)pd-l1阻斷
免疫熒光
免疫組化（冷凍）
流式細胞術(shù)
蛋白質(zhì)印跡
配方pbs，ph 6.5
不含穩(wěn)定劑或防腐劑
內(nèi)毒素<2eu/mg （<0.002eu/μg）
通過lal凝膠凝血測定法測定
純度>95%
由sds-page 決定
無菌0.2 μm 過濾
生產(chǎn)從無動物設(shè)施中的組織培養(yǎng)上清液中純化
純化蛋白g
里德ab_10949073
分子量150 千達
存儲抗體溶液應(yīng)以4°c的儲備濃度儲存。 不要凍結(jié)。